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tissue pge2 levels  (Elabscience Biotechnology)


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    Elabscience Biotechnology tissue pge2 levels
    Insulin injection increased phospho-ERK expression but not phospho-cPLA2 expression in male mice. ( a ) Representative images of phospho-ERK (p-ERK) immunohistochemistry in the hypothalamus 60 min after i.p. injecton of saline ( n = 4) or 0.75 U/kg insulin ( n = 3). ( b ) Quantification of p-ERK expression in the hypothalamus; ARC: arcuate nucleus; VMH: ventromedial hypothalamus. ( c ) Representative images of phospho-cPLA2 (p-cPLA2) immunohistochemistry in the hypothalamus 60 min after i.p. injection of saline ( n = 4) or 0.75 U/kg insulin ( n = 4). ( d ) Quantification of p-ePLA2 expression in the hypothalamus. ( e ) <t>Prostaglandin</t> <t>E2</t> levels in the hypothalamus of shSCRM ( n = 4) or shPLA2 ( n = 5) injected mice. Arrowhead: p-ERK immunoreactivity. Scale bars: 200 µm. All data represent the mean ± SEM; **** p < 0.0001. The unpaired Student’s t -test (two-tailed p -value) was used for statistical analysis.
    Tissue Pge2 Levels, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 293 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tissue+pge2+levels/pmc12347649-138-0-10?v=Elabscience+Biotechnology
    Average 96 stars, based on 293 article reviews
    tissue pge2 levels - by Bioz Stars, 2026-07
    96/100 stars

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    1) Product Images from "Suppression of Cytosolic Phospholipase A2 in the Ventromedial Hypothalamus Induces Hyperphagia and Obesity in Male Mice"

    Article Title: Suppression of Cytosolic Phospholipase A2 in the Ventromedial Hypothalamus Induces Hyperphagia and Obesity in Male Mice

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms26157532

    Insulin injection increased phospho-ERK expression but not phospho-cPLA2 expression in male mice. ( a ) Representative images of phospho-ERK (p-ERK) immunohistochemistry in the hypothalamus 60 min after i.p. injecton of saline ( n = 4) or 0.75 U/kg insulin ( n = 3). ( b ) Quantification of p-ERK expression in the hypothalamus; ARC: arcuate nucleus; VMH: ventromedial hypothalamus. ( c ) Representative images of phospho-cPLA2 (p-cPLA2) immunohistochemistry in the hypothalamus 60 min after i.p. injection of saline ( n = 4) or 0.75 U/kg insulin ( n = 4). ( d ) Quantification of p-ePLA2 expression in the hypothalamus. ( e ) Prostaglandin E2 levels in the hypothalamus of shSCRM ( n = 4) or shPLA2 ( n = 5) injected mice. Arrowhead: p-ERK immunoreactivity. Scale bars: 200 µm. All data represent the mean ± SEM; **** p < 0.0001. The unpaired Student’s t -test (two-tailed p -value) was used for statistical analysis.
    Figure Legend Snippet: Insulin injection increased phospho-ERK expression but not phospho-cPLA2 expression in male mice. ( a ) Representative images of phospho-ERK (p-ERK) immunohistochemistry in the hypothalamus 60 min after i.p. injecton of saline ( n = 4) or 0.75 U/kg insulin ( n = 3). ( b ) Quantification of p-ERK expression in the hypothalamus; ARC: arcuate nucleus; VMH: ventromedial hypothalamus. ( c ) Representative images of phospho-cPLA2 (p-cPLA2) immunohistochemistry in the hypothalamus 60 min after i.p. injection of saline ( n = 4) or 0.75 U/kg insulin ( n = 4). ( d ) Quantification of p-ePLA2 expression in the hypothalamus. ( e ) Prostaglandin E2 levels in the hypothalamus of shSCRM ( n = 4) or shPLA2 ( n = 5) injected mice. Arrowhead: p-ERK immunoreactivity. Scale bars: 200 µm. All data represent the mean ± SEM; **** p < 0.0001. The unpaired Student’s t -test (two-tailed p -value) was used for statistical analysis.

    Techniques Used: Injection, Expressing, Immunohistochemistry, Saline, Two Tailed Test



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    Insulin injection increased phospho-ERK expression but not phospho-cPLA2 expression in male mice. ( a ) Representative images of phospho-ERK (p-ERK) immunohistochemistry in the hypothalamus 60 min after i.p. injecton of saline ( n = 4) or 0.75 U/kg insulin ( n = 3). ( b ) Quantification of p-ERK expression in the hypothalamus; ARC: arcuate nucleus; VMH: ventromedial hypothalamus. ( c ) Representative images of phospho-cPLA2 (p-cPLA2) immunohistochemistry in the hypothalamus 60 min after i.p. injection of saline ( n = 4) or 0.75 U/kg insulin ( n = 4). ( d ) Quantification of p-ePLA2 expression in the hypothalamus. ( e ) <t>Prostaglandin</t> <t>E2</t> levels in the hypothalamus of shSCRM ( n = 4) or shPLA2 ( n = 5) injected mice. Arrowhead: p-ERK immunoreactivity. Scale bars: 200 µm. All data represent the mean ± SEM; **** p < 0.0001. The unpaired Student’s t -test (two-tailed p -value) was used for statistical analysis.
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    Insulin injection increased phospho-ERK expression but not phospho-cPLA2 expression in male mice. ( a ) Representative images of phospho-ERK (p-ERK) immunohistochemistry in the hypothalamus 60 min after i.p. injecton of saline ( n = 4) or 0.75 U/kg insulin ( n = 3). ( b ) Quantification of p-ERK expression in the hypothalamus; ARC: arcuate nucleus; VMH: ventromedial hypothalamus. ( c ) Representative images of phospho-cPLA2 (p-cPLA2) immunohistochemistry in the hypothalamus 60 min after i.p. injection of saline ( n = 4) or 0.75 U/kg insulin ( n = 4). ( d ) Quantification of p-ePLA2 expression in the hypothalamus. ( e ) <t>Prostaglandin</t> <t>E2</t> levels in the hypothalamus of shSCRM ( n = 4) or shPLA2 ( n = 5) injected mice. Arrowhead: p-ERK immunoreactivity. Scale bars: 200 µm. All data represent the mean ± SEM; **** p < 0.0001. The unpaired Student’s t -test (two-tailed p -value) was used for statistical analysis.
    Prostaglandin E2 Colon Tissue Pge2 Levels, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Insulin injection increased phospho-ERK expression but not phospho-cPLA2 expression in male mice. ( a ) Representative images of phospho-ERK (p-ERK) immunohistochemistry in the hypothalamus 60 min after i.p. injecton of saline ( n = 4) or 0.75 U/kg insulin ( n = 3). ( b ) Quantification of p-ERK expression in the hypothalamus; ARC: arcuate nucleus; VMH: ventromedial hypothalamus. ( c ) Representative images of phospho-cPLA2 (p-cPLA2) immunohistochemistry in the hypothalamus 60 min after i.p. injection of saline ( n = 4) or 0.75 U/kg insulin ( n = 4). ( d ) Quantification of p-ePLA2 expression in the hypothalamus. ( e ) Prostaglandin E2 levels in the hypothalamus of shSCRM ( n = 4) or shPLA2 ( n = 5) injected mice. Arrowhead: p-ERK immunoreactivity. Scale bars: 200 µm. All data represent the mean ± SEM; **** p < 0.0001. The unpaired Student’s t -test (two-tailed p -value) was used for statistical analysis.

    Journal: International Journal of Molecular Sciences

    Article Title: Suppression of Cytosolic Phospholipase A2 in the Ventromedial Hypothalamus Induces Hyperphagia and Obesity in Male Mice

    doi: 10.3390/ijms26157532

    Figure Lengend Snippet: Insulin injection increased phospho-ERK expression but not phospho-cPLA2 expression in male mice. ( a ) Representative images of phospho-ERK (p-ERK) immunohistochemistry in the hypothalamus 60 min after i.p. injecton of saline ( n = 4) or 0.75 U/kg insulin ( n = 3). ( b ) Quantification of p-ERK expression in the hypothalamus; ARC: arcuate nucleus; VMH: ventromedial hypothalamus. ( c ) Representative images of phospho-cPLA2 (p-cPLA2) immunohistochemistry in the hypothalamus 60 min after i.p. injection of saline ( n = 4) or 0.75 U/kg insulin ( n = 4). ( d ) Quantification of p-ePLA2 expression in the hypothalamus. ( e ) Prostaglandin E2 levels in the hypothalamus of shSCRM ( n = 4) or shPLA2 ( n = 5) injected mice. Arrowhead: p-ERK immunoreactivity. Scale bars: 200 µm. All data represent the mean ± SEM; **** p < 0.0001. The unpaired Student’s t -test (two-tailed p -value) was used for statistical analysis.

    Article Snippet: Tissue PGE2 levels were determined by ELISA (PGE2 ELISA Kit, Elabscience, Houston, TX, USA), with absorbance measured at 450 nm using a microplate reader (SH9000Lab, Corona Electric, Hitachinaka, Japan).

    Techniques: Injection, Expressing, Immunohistochemistry, Saline, Two Tailed Test